27 May 2026, Elkhart: Agdia, Inc. is pleased to announce the commercial launch of a field-deployable rapid molecular test kit on its AmplifyRP® XRT platform for onsite detection of Cucumber vein yellowing virus (CVYV).
Growers can also send samples to Agdia’s Testing Services diagnostic laboratory for expanded cucurbit pathogen screening that now includes CVYV testing.
Figure 1. Pair the new test with Agdia’s AmpliFire® Pro portable isothermal fluorometer (coming soon)
Cucumber vein yellowing virus is a plant pathogenic virus from the Ipomovirus genus that primarily causes disease in cucurbits, but can also infect several other crops, including weed species that act as natural host reservoirs. The virus has been detected primarily in the Mediterranean region, with reports from countries including Cyprus, Iran, Israel, Jordan, Portugal, Spain and Turkey. It is transmitted by the silverleaf whitefly (Bemisia tabaci) and mechanical means such as pruning.
While CVYV can infect various weed species, several cucurbit species are of primary economic concern, including cucumber (parthenocarpic cucumbers in particular), melon, squash and watermelon.
Symptoms can vary by host species but generally include:
Chlorosis
Vein clearing
Stunting
Necrosis
Fruit cracking or splitting
Some of these symptoms are similar in appearance to other viruses and nutrient deficiency issues, making accurate diagnosis essential to effective crop protection.
Extensive validation studies were conducted to demonstrate fitness-for-purpose of the new molecular assay developed by Agdia for the detection of CVYV:
Diagnostic specificity is 100% (58 out of 58 true negatives detected). No cross reactivity was observed with other pathogens tested during product validation.
The new assay successfully detected 119 out of 121 positive samples, resulting in a diagnostic sensitivity of 98.3%.
This test detects (or is predicted to detect via in silico analysis) all known isolates from various geographic regions, including Israel, Lebanon, Spain, Portugal, Jordan, Oman and Sudan.
To view the full validation report, see the “Technical Information” tab on the product page.
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